Expression levels of genes of NKG2C+ NK cells after in vitro treatment

RNA-Seq and comparison of gene expression levels was performed of FACS-sorted viable CD56+ NKG2C+ NK cells after in vitro treatment with peptide-pulsed RMA-S/HLA-E/LFA-3 target cells in the presence of or absence of IL-12 and IL-18 NK cells of HCMV-seronegative healthy individiuals were FACS-sorted for viable CD3- CD56dim cells. CD56dim NK cells were co-cultured for 7 days with RMA-S/HLA-E/LFA-3 target cells either pulsed with VMAPQSLLL or VMAPRTLFL peptide in the presence of 10 ng/mL IL-15. 10 ng/mL IL-12 and 100 IL-18 were either present or not during the initial 20 h of culture.