Crystal structure of the polyester hydrolase Leipzig 7 (PHL7) mut3 variant with glycosylation by expression in Pichia pastoris (P_PHL7mut3)

Crystal structure of the polyester hydrolase Leipzig 7 (PHL7) mut3 variant with glycosylation by expression in Pichia pastoris (P_PHL7mut3) Descriptor: 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, Polyester Hydrolase Leipzig 7 (PHL-7), catalysis-deficient S131A mutant, ... Authors: Useini, A, Strater, N, Striedner, G, Sonnendecker, C. Deposit date: 2025-03-03 Release date: 2025-09-10 Last modified: 2025-12-03 Method: X-RAY DIFFRACTION (1.37 Å) Cite: Trade-Offs between Stability and Activity of Glycosylated and Non-Glycosylated Polyester Hydrolases PHL7 and PHL7mut3. Acs Es T Eng, 5, 2025在毕赤酵母（Pichia pastoris）中表达并经糖基化修饰的聚酯水解酶Leipzig 7（PHL7）mut3变体（P_PHL7mut3）的晶体结构。描述项：2-乙酰氨基-2-脱氧-β-D-吡喃葡萄糖-(1-4)-2-乙酰氨基-2-脱氧-β-D-吡喃葡萄糖、聚酯水解酶Leipzig 7（PHL-7）的催化缺陷型S131A突变体……。作者：Useini A、Strater N、Striedner G、Sonnendecker C。提交日期：2025-03-03，发布日期：2025-09-10，最后修改日期：2025-12-03。检测方法：X射线衍射（X-RAY DIFFRACTION），分辨率1.37埃（Å）。引用文献：《糖基化与非糖基化聚酯水解酶PHL7及PHL7mut3的稳定性与活性权衡》，Acs Es T Eng，5，2025