Sciatic nerve crush regulation of RNA alternative poly-adenylation [DRGinjury_2019-QuantSeq_Rev_24h]

Poly-adenylation regulates post-transcriptional processing and localization of mRNA. To study the effect of nerve injury on poly-adenylation across the transcriptome, 3'-end RNAseq was carried on DRG neurons following sciatic nerve injury. Mice were subjected to crush of the sciatic nerve in one side, while the other nerve was spared and used as naive control condition. Overall design: Mice were subjected to crush injury on in one sciatic nerve. Corresponding DRG (Lumbar L4-L6) were dissected 24 h after injury and total RNA was extracted using Qiagen RNEasy Mini kit. Injured and naive (contralateral side) ganglia were extracted from two mice per repeat.