Evaluation of the effect of storage solutions on the bond strength to the dentin of bovine teeth under micro-incision – in vitro research

Abstract Introduction With the difficulty of obtaining human teeth for in vitro studies, authors report and recommend the use of teeth extracted from bovine arches to test techniques from different segments of Dentistry. However, for research to be scientifically effective, these teeth need adequate treatment to maintain their characteristics and to mimic conditions in vivo. Objective Analyze the adhesive bond strength (RU) to dentin in bovine teeth restored with Adper® Single Bond 2 (3M ESPE, St Paul, MN, USA) and Opallis Flow composite adhesive (Dentscare LTDA, Joinville, SC, BRA), taking as study factors solution and storage time. Methodology The samples were frozen under storage in 2 solutions: 0.1% thymol and 0.5% chloramine, in 4 different periods: 1 week, 1 month, 3 months and 6 months. Eight experimental groups (n = 64) and one control group (n = 8) were obtained, totaling 9 groups. After the restoration period, the teeth were stored in an incubator at 37ºC, immersed in distilled water and then tested in a universal testing machine (EMIC DL 500, São José dos Pinhais, PR, Brazil), with statistical analysis of the results. Result The comparison between the experimental and control groups showed a statistically significant difference (p <0.05) for the storage of thymol samples during the 1 month period. Conclusion Solution and time factors were able to change the adhesive bond strength to the bovine dentin, observing that the highest mean of RU was obtained after storage in the thymol solution for 1 month (p = 0.004).