The use of ESSENTIALS on data generated by various transposon sequencing techniques.

$Optimal fold change (FC) underrepresentation cut-offs detected by ESSENTIALS; N: number; NA: Not available, a different method was used to determine gene essentiality in these studies. #A minimum normalized average read count of 50 reads per gene was required; FC cut-offs were the same as used in the literature reference to facilitate comparison. *Although the authors state in their methods that a −2 log2 fold change and a p<1*10-5, adj. p<2.5E-4 cut-off was used, only the p-value cut-offs were applied, resulting in a -1.4 fold change cut-off (personal communication Julian Parkhill).