Nanos-2 mutant line of the sea anemone Nematostella vectensis

CRISPR/Cas9 mediated gene knockout show that nanos2+ progenitor cells are indispensable for male and female germline maintenance in Nematostella. This suggests nanos and piwi genes have a conserved role in somatic and germline stem cells in cnidarians. Embryos/animals were dissociated and cell suspensions were processed with a 10X Genomics Chromium Controller