Julien Mouysset, Alexandra Deichsel, Sandra Moser, Carsten Hoege, Anthony A. Hyman, Anton Gartner, Thorsten Hoppe (2011) CIL:35123, Caenorhabditis elegans, early embryonic cell. CIL. Dataset

This time lapse series illustrates the early division up to the four-cell stage of a C. elegans embryo expressing YFP::CDC-48 by DIC and fluorescence microscopy. YFP::CDC-48, like YFP::UFD-1 and YFP::NPL-4, are cytosolic during mitosis, but accumulate in the nucleus after its reformation. The first C. elegans embryonic division of the P0 zygote is asymmetric and generates an anterior AB cell, and a smaller posterior P1 cell. These cells have different developmental fates and division timing, with AB dividing approximately 2 min before P1. Additionally, in early C. elegans embryonic cell cycles, S and M phases rapidly alternate without apparent gap phases. The movie corresponds to experiments shown in Fig. 1A and Fig. S1 and Movie 1 of Mouysset et al. Movie is CIL 25628 and original data file is CIL 35123.

本时序系列图展示了秀丽隐杆线虫胚胎在表达YFP::CDC-48蛋白的情况下，通过相差干涉和荧光显微镜观察到的从早期分裂至四细胞期的过程。YFP::CDC-48蛋白与YFP::UFD-1和YFP::NPL-4蛋白类似，在细胞有丝分裂期间位于细胞质中，但在分裂重建后聚集于细胞核。P0合子的第一个秀丽隐杆线虫胚胎分裂是不对称的，产生了位于前端的AB细胞和较小的后端P1细胞。这些细胞具有不同的发育命运和分裂时间，其中AB细胞大约在P1细胞分裂前2分钟进行分裂。此外，在秀丽隐杆线虫胚胎的早期细胞周期中，S期和M期快速交替，无明显间隙期。该视频与图1A、图S1及Mouysset等人的Movie 1中的实验相对应。视频编号为CIL 25628，原始数据文件编号为CIL 35123。