16S amplicon sequencing of bacteria from two entomopathogenic nematodes, along with their nematode-free hosts, as negative controls. Targeted loci. Drosophila falleni (or associated parasitic nematode Howardula aoronymphium) and Spelobia sp. (or unnamed associated nematode)

We have sequenced the V3V4 hypervariable region of the 16S rRNA gene from four distinct sources. 1) Drosophila falleni; 2) Howardula aoronymphium (parasite of D. falleni); 3) Spelobia sp.; and 4) parasitic nematode of Spelobia sp. The goal of this work was to determine whether a newly described clade of endosymbiotic bacteria, Symbiopectobacterium, is found in Howardula or its host, and whether closely related nematodes (in Spelobia) harbour similar symbionts.