Human Microbiome Project (HMP) 16S rRNA Gene Diversity, the diversity of 16S ribosomal RNA genes in the human microbiome: 454 Protocol Validation - Clinical. human metagenome

This HMP Centers' evaluation of the default 454 SOP represents the pyrosequencing of 16S rRNA genes amplified from anonymized clinical stool samples distributed to each of the four HMP sequencing centers. The goal of the pilot was to test the provisional 454 sequencing protocol (v4.2) and to evaluate accuracy and consistency between centers. (Previously referred to as Centers Evaluation of 454 SOP (CEFoS) Clinical Pilot)