RBM22 CUT&Tag Profiling of Regenerative (P1) and Non-Regenerative (P8) Neonatal Mouse Cardiomyocytes

Purpose: To uncover the molecular mechanisms underlying RBM22-mediated neonatal heart regeneration, we performed Cleavage Under Targets and Tagmentation (CUT&Tag) analysis on regenerative and non-regenerative wild-type (WT) mouse cardiomyocytes over a 7-day period. Methods: Cardiomyocytes were isolated from postnatal day 1 (P1, regenerative) and postnatal day 8 (P8, non-regenerative) mice, and cultured in DMEM supplemented with 10% fetal bovine serum at 37°C in a 5% CO2 atmosphere. Cells were stained to confirm the expression of cTnT, and only those with purity >97.5% were used for subsequent experiments.Results: We carried out a CUT&Tag assay using antibody against RBM22 and found the global influence of RBM22 in regulating genes in transcription regulatory regions of key genes involved in proliferative cardiomyocyte effector function. Overall design: Cleavage Under Targets and Tagmentation (CUT&Tag) analysis for RBM22-binding DNA in P1 (regenerative) and P8 (non-regenerative) wild-type (WT) mouse cardiomyocytes.