Genotyping of 180 feathers of Golden eagle from french mountain massifs

Two batches of 90 feathers of Golden eagle were genotyped. The first batch included 90 feathers collected during four consecutive years in four golden eagle territories of the Massif Central. Of these feathers, 19 were directly sampled from individuals (adults and chicks, see appendix A) and 71 were collected from nests. 

A second batch of 90 feather samples collected from nests or directly from individuals in five different French mountainous regions was performed: Drôme (N=13), Vanoise (N=4), Hautes-Alpes (N=22), Massif Central (N=40) and Pyrenees (N=11). 

These two batches of feathers were genotyped on 17 microsatellite markers divided into two multiplexes: A (8 markers) and B (9 markers). These markers were selected from several past studies (Gautschi et al. 2000; Hailer et al. 2005; Martinez-Cruz et al. 2005; Bourke and Dawson 2006; Bielikova et al. 2010).