Transcriptome analysis of 16HBE cell line models harbouring homozygous Cystic Fibrosis causing mutations

The aim of this study was to generate a comparative overview of the impact of different CF-causing mutations on the transcriptome of human bronchial epithelial cells (16HBE14o-). For this purpose, we performed paired-end Illumina mRNA-Seq from total RNA samples isolated from WT and CRISPR gene edited cell lines carrying different homozygous CFTR mutations (F508del, N1303K, G551D and I1234V), using three independent biological replicates.