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SLAMseq in mouse model of acute liver injury

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NIAID Data Ecosystem2026-05-02 收录
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https://www.ncbi.nlm.nih.gov/sra/ERP159908
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Extracellular RNA (exRNA) mediates intercellular communication in plants and lower animals; whether it serves a signalling function in mammals is controversial. We sought to determine the scale of RNA transfer between liver and kidney through the metabolic labelling of RNA in mice. We used 4-thiouracil to specifically label RNA in hepatocytes and SLAMseq (SH-Linked Alkylation for the Metabolic sequencing of RNA) to detect labelled (thiolated) RNA in liver and kidney.
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2024-07-28
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