Plasma exosomes miRNAs high-throughput sequencing in patients after STEMI 3-6 months

ST-segment elevated myocardial infarction (STEMI) is one of the most severe forms of cardiovascular heart diseases. In the present study, we aim to describe differential expressed plasma exosome-miRNAs in patients with post-STEMI 3-6 months. Methods: Consecutive patients with ages from 40 to 80 years and 25 males among them 3-6 months after STEMI (n=11) were compared to sex-matched healthy control (n=10). The mean age of the patient was 64.71±10.40 years and 89.2% were male. Compared to the healthy control group, the plasma exosome-miRNAs were assessed by using microarray assay (IIIumnia Hiseq 2500). Profile of plasma exosome-miRNAs related to heart diseases was established both in the patient and control groups. The specificity of the lowest expressed exosome-miRNAs for all subjects was evaluated by using a quantitative real-time polymerase chain (qPCR). plasma exosomes miRNAs were obtained from STEMI patients after 3-6 months and healthy subjects Overall design: we explored the plasma exosomes miRNAs profile of 10 STEMI after 3-6 months, and 10 healthy individuals by RNA sequencing analyses to investigate its features and potential targets for postponing myocardium remodeling.