Single Cell RNA Sequencing of Monocyte Derived Endothelial Cells

We examined the global transcriptome of individual cells in the three cell populations, starting MC from three donors after 1hr adherence to FN pooled, MC-derived EC subjected to shear stress, and HCAEC subjected to shear stress using single cell RNA-sequencing (scRNA-Seq). Analysis showed that the initial population of MC clustered separately from HCAEC, while the resulting MCEC overlapped with HCAEC and not MC. Co-expression analysis reveals that only the resulting MCEC contain cells co-expressing KDR and PROM1 (CD133), whereas our starting MC population contained only one cell expressing KDR and a separate cell expressing PROM1 suggesting that our initial MC population did not contain any EPC/ECFCs. Interestingly, heatmaps constructed using EC and MC associated genes revealed that the resulting MCEC cells expressed predominantly EC genes but also some MC genes. Examination of global transcriptome of 3 cell populations, initial monocytes, monocyte derived endothelial cells, and human endothelial cells as a control