The Transcriptome Analysis of THP-1 Macrophage Infected with ΔM5447 or Wt Mycobacterium smegmatis by Next Generation Sequencing

Purpose: The goals of this study are systematic analysis of cellular pathway in THP-1 Macrophage which infected with wild type mycobacterium smegmatis (Wt) or MSMEG_5447 gene defected Mycobacterium smegmatis( ΔM5447) at 24h postinfection. Method: THP-1 Macrophages mRNA profiles infected with Wt orΔM5447 at 24h postinfection were generated by deep sequencing, in duplicate, using Illumina HiSeq platform and human transcriptome (RefSeq transcriptome index hg19) is a reference sequence. Results: In total, 497 differentially expressed genes (DEGs) was identified in ΔM5447-infected macrophages relative to the Wt-infected macrophages which including 173 up-regulated genes and 324 down-regulated genes using adjusted p-value<0.05 as the threthold criteria. The DEGs of THP-1 macrophages infected with ΔM5447 strain was enriched in inflammatory related pathway. Conclusion: Our study find that the THP-1 macrophages infected with ΔM5447 strain display decreased inflammatory response, MSMEG_5447 may play an essential role in inflammatory response. THP-1 Macrophages mRNA profiles infected with Wt orΔM5447 at 24h postinfection were generated by deep sequencing, in duplicate, using Illumina HiSeq, platform, Wt as control sample.