RIP-seq analysis of Isw1 interacting transcripts

This experiment captures the poly(A)RNAs co-immunoprecipated with Isw1 in WT and npl3-1 cells. Chromatin remodelers play an essential role in regulating DNA transaction processes. The yeast chromatin remodeling complex ISW1 displays an unexpected function in nuclear mRNP surveillance, retaining premature mRNPs in proximity to their transcription site. This activity of ISW1 requires its recruitment onto chromatin, its interaction with mRNPs but not its nucleosome sliding activity. In npl3-1 cells, mRNA export is compromised resulting in an increased association of mRNAs with Isw1.