neuroendocrine control of planarian regeneration (Spatial transcriptome)

Effective tissue regeneration requires cells to perceive the surrounding context and make internal state adaptations that enable context-dependent fate changes in time and space, but the mechanisms underlying this complex process remain unclear. Here, transcriptom sequencing was used for study neuroendocrine control of planarian regneration Overall design: Examine gene expression profiles of regenerated asexual planarian fragments after amputation at different time points, wild type intact asexual planarian and 3dpa tail after RNAi. Stereo-seq data for six time points (0h, 12h, 1.5d, 3d, 5d, 10d) and the 10X Visium data for five time points (0h, 12h, 1.5d, 3d, 5d). Please note that the 'regeneration (mixed sample)' raw data are non-demultiplexed files generated from all samples (5 time-point; 0h, 12h, 1.5d, 2.5d, 5d) and the details of splited 5 time-point samples are included in '10X_Visium_filtered_feature_bc_matrix_and_image.zip'. Schmidtea mediterranea include both asexual and sexual types. Our experiments used asexual Schmidtea mediterranea (strain CIW4) animals, and sequencing data were matched using transcriptome (GSE72389_smed_20140614.fa.gz).