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Promixity labling and Affinity purification of TRIAD3A interactome

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NIAID Data Ecosystem2026-05-02 收录
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To identify cargo content involved in TRIAD3A-mediated selective autophagy, we expressed TRIAD3A fused to an engineered biotin ligase (TurboID) by viral transduction in mouse brain excitatory neurons (AAV-CAMKII-TRIAD3A-V5-TurboID). After treatment with biotin substrate, brain was harvested and biotinylated proteins were identified by liquid chromatography-tandem mass spectrometry

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2024-06-14
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