RNA-Seq analysis of murine pro-B BAF3 cells transfected with oncogenic form of receptor tyrosine kinases

To identify gene expression changes associated with receptor tyrosine kinase (RTK) activation, we performed RNA-Seq analysis of murine pro-B BAF3 cells over-expressing the oncogenic form of EGFR (EGFR-L858R-T790M), FGFR (TEL-FGFR1 fusion), MET (TPR-MET fusion) or RET (CCDC6-RET fusion) genes. We reported the RTK-driven transcriptional reprogramming of metabolic genes. Overall design: Murine pro-B BAF3 cells were stably transfected with EGFR-L858R-T790M, TEL-FGFR1 fusion, TPR-MET fusion or CCDC6-RET fusion. The gene expression change versus BAF3 parental cells were analyzed using RNA-seq analysis. Numbers of significantly activated genes in different RTK-driven cells had been identified.