Rattus norvegicus Transcriptome or Gene expression. Rattus norvegicus
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Total RNA was isolated using the TRIzol Reagent (Beyotime Biotechnology Corporation) and quantified using a NanoPhotometer® spectrophotometer (IMPLEN, CA, USA). A total amount of 3 µg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumia) according to the manufacturer’s instructions. After cluster generation, the library preparations were sequenced on an Illumina platform and 125 bp/150 bp paired-end reads were generated. Sequences obtained from the RNASeq pipeline were aligned against the Rattus norvegicus genome using STAR.
