Supplemental Material for Deng et al.

Supplemental Table S1. Strains used
in this work.

Supplemental Table S2. List of
conserved kinases screened to find new negative regulators of LIN-12/Notch. Validated positive hits are highlighted in blue. The
WormBase ID (WBgene), common name and locus ID for the C. elegans kinases
screened are shown in the first three columns. The fourth column shows either
(i) feeding-RNAi clones obtained from the commercially available “Ahringer
Library” (Kamath and Ahringer 2003), (ii) clones derived from the “Vidal
Library” (Rual et al. 2004), and (iii) clones generated by us to fix those
that were not correct in the Ahringer Library (against daf-2,
gck-1 and T01G5.1), and supplemental clones that did targeted all
predicted isoforms of certain genes (atl-1, pkc-1) or did not exist in
either the Ahringer or Vidal libraries. The bottom of the table shows clones
that targeted five kinase-encoding genes that are now considered pseudogenes (www.wormbase.org, ver. WS272).

Supplemental Table S3. Primers used
to make RNAi clones. We used these primers to
PCR amplify fragments from genomic DNA that encompass exonic regions to target
kinases. Most of the primers, and resulting PCR products, described here were
made to generate clones against kinases that were not represented in either of
the available “feeding” RNAi libraries (see main text). Three of the PCR
products were used to correct erroneous clones (daf-2, gck-1 and T01G5.1),
or to create new clones that would target kinase isoforms that were missed by
previously existing clones (atl-1 and pkc-1). The
bottom portion of the table shows primers used to build second, or third,
“non-overlapping” RNAi clones to confirm results from the initial screen for
those genes where null alleles were unavailable or caused lethality (see Fig.
3, main text).

Supplemental Figure S1. Additional biological replicate testing enhancement of lin-12(n302) as
compared to lin-12(+).