Gene expression under normoxia and hypoxia in mouse embryonic fibroblasts. Mus musculus

Mouse and human somatic cells can be reprogrammed to induced pluripotent stem cells (iPSCs) by the transduction of four transcription factors, Oct 3/4, Sox2, Klf4, and c-Myc. Conducting reprogramming in hypoxic conditions was shown to result in improved efficiency for both mouse and human cells. To examine the effect of hypoxic cultivation, we performed microarray analyses using mouse embryonic fibroblasts cultivated under normoxia and hypoxia. Overall design: Mouse embryonic fibroblasts and four factor-transduced reprogramming fibroblasts were grown under normoxia and hypoxia. These cells were used for microarray analyses.