Transcriptional remodeling by OTX2 directs specification and patterning of mammalian definitive endoderm [PS_CUTnRUN]

We discovered a role for the transcription factor OTX2 in formation of mouse definitive endoderm (DE). We used CUT&RUN to map OTX2-bound genomic regions and identify OTX2-regulated genes during directed differentiation of EpiSCs to DE EpiSCs were converted to DE and live cells isolated by flow cytometry. OTX2 binding sites were mapped in mouse EpiSCs, Primitive Streak (PS), and definitive endoderm using CUT&RUN for HA in OTX2-HA tagged cell lines.