Single cell analysis of tumors developed from mouse dysplastic stem cells

To investigate whether the CD44v6neg/CD133+/CD166+ dysplastic stem cells are responsible for gastric adenocarcinoma development and cancer cell heterogeneity in the stomach, we used single cell RNA-seq (scRNA-seq) to examine gene expression profiles of heterogenous cancer cells in two types of gastric adenocarcinoma, cystic type and tubular type, developed from the dysplastic stem cell injection under the skin of immunodeficient nude mice. Overall design: Cells were dissociated from fresh Cystic or tubular type of gastric adenocarcinoma tumors and encapsulated for encapsulation