Genome-wide map of chromatin states and global transcriptome profiling in SETD2 wild type and mutant cells

We looked at the distribution of the histone mark H3K36me3 in wild type 293T and SETD2 deleted 293T (KO) cells. We also looked at the H3K36me3 profile after rescuing the KO cells with recombinant SETD2 constructs; FL (full length), N3 (1404-2564) and N3?SRI/N3woSRI (1404-2468). Furthermore, we performed RNA-Seq of cells expressing the wild type or mutant/recombinant copy of SETD2 to compare their global transcriptome profile. Overall design: Wild type, SETD2 deleted (KO) and KO cells rescued with recombinant SETD2 constructs; FL (full length), N3 (1404-2564) and N3?SRI/N3woSRI (1404-2468) were used in the study. The KO cells in addition to being the cells in which rescue experiments with recombinant SETD2 were performed, also serve as a negative control for H3K36me3 ChIP.