Chemotactic response of St-T1b cells or primary hESCs to PDGF-BB, HB-EGF and trophoblast conditioned medium.

St-T1b cells (upper panel) or hESC (lower panel), non-decidualized (ND) or decidualized (D) by 5 d treatment with 8-Br-cAMP/MPA, were subjected to transwell migration assay. The bottom reservoir contained MM1-10% (controls), PDGF-BB, HB-EGF or conditioned medium (TCM) from the trophoblast cell line AC-1M88 undiluted (100%) or diluted to 80% or 20%. The motility index designates the percentage of migrated cells relative to the total cell number (non-migrated plus migrated) at the end of the 18 h migration period. Shown are results representative of 3 similar experiments (means± SD, n = 3). Data were analyzed by ANOVA and Dunnett post-hoc test. *, P<0.05; **, P<0.01; *** P<0.001 compared to the respective control within the ND or D groups.