Expression data from PrimeView human gene expression array

Gene expression in HCT-116 cells infected with shCtrl and shRFWD3 was detected with a PrimeView human gene expression array. We performed Affymetrix Human Gene Chip Prime View analysis on shCtrl and shRFWD3 HCT 116 cells. Of the 1305 differentially expressed genes (DEGs) found in shRFWD3 and shCtrl HCT 116 cells, 629 were up-regulated and 676 were down-regulated based on the threshold of absolute fold change ≥ 2 and FDR < 0.05 The total RNA of HCT-116 cells infected with shCtrl and shRFWD3 was extracted. The RNA sequencing was performed with Affymetrix human GeneChip PrimeView and the outcomes were scanned by Affymetrix Scanner 3000. The statistical significance of raw data was completed by a Welch t-test with Benjamini-Hochberg FDR (|Fold Change| ≥ 2 and FDR < 0.05 as significant).