Analysing the gene expression between wildtype and Nxt1 trans-heterozygotes in whole larvae

We used whole larvae of both wildtype and Nxt1 trans-heterozygotes for RNA paired-end sequencing and obtained a read depth between 6.4M to 11.1M reads across all samples. In wandering larvae, stationary larvae and white prepupae, a total of 435, 855, and 611 genes were differentially expressed. A high percentage of down-regulated genes were highly expressed/testis-specific in the Drosophila testis. Examination of gene expression in 2 different genotypes, WT and Nxt1 trans-heterozygotes.