Inflammatory crosstalk impairs phagocytic receptors and aggravates atherosclerosis in clonal hematopoiesis in mice

Low frequency Jak2VF mutations promote atherosclerosis via IL-1 mediated cross-talk. Therapeutic approaches that increase MERTK or TREM2 could promote plaque stabilization in inflammasome-driven atherosclerosis. To determine if activating TREM2 could improve atherosclerosis in LAB Jak2VF CH mice, we administered 4D9 or isotype control IgG antibodies by i.p. injection beginning 2 weeks after initiation of the WD and continued injections with the diet for an additional 14 weeks. Digested live aortic cells were used for the Singe cell RNA-Seq analysis.