Effects of kockouts of EGR1, FOS and their combination on the MAPK induced transcriptome [RNA_KO_clones]

In this study we used CRISPR/Cas9 to generate KO clones of the HEK-RAF-ER cell line that lack expression of EGR1, FOS and profiled the transcriptome after inducing RAF/MAPK signaling. Overall design: We generated multiple KO clones of EGR1 and FOS using guides that target the promoter and the first exon. From these clones, we also generated combinatorial KOs. We then profiled the transcriptome before and 6h-18h after induction of RAF/MAPK in those KO clones and in selected WT clones.