Wound Healing Assays in NSD3short Overexpressing H1299 Cells
收藏NIAID Data Ecosystem2026-05-02 收录
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https://zenodo.org/record/1226773
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SGC Open Notebook Project to Characterize the HMTase NSD3
Exp022 Objective: During epithelial to mesenchymal transitioning (EMT), transcription networks become activated and cytoskeletal rearrangements take place. These events promote migratory capacity and invasiveness. A common assay to measure this phenotype is wound healing. This involves plating cells and culturing until confluent at which point a scratch or wound is made in the culture. This area is imaged over a 24-72 period to monitor the rate of wound closure. Cells with increased migratory capacity will close the wound faster and are indicative of a more mesenchymal state
创建时间:
2024-08-02



