Coordinate regulation of ammonium transporters and components of the mating and pathogenic programs in Ustilago maydis. Mycosarcoma maydis

Next generation sequencing was used to assess transcriptome changes in haploid U. maydis wild type and mutant strains grown under nitrogen-replete and low ammonium media. Overall design: Wild type and mutant strains were grown for 48 hours at 26 C, in microarray media ((6.25% Holliday Salt Solution (Holliday, 1974), 1% glucose, and 2% agar) with either 30 mM Glutamine/50 mM Ammonium sulfate (Hi) or 50 uM Ammonium sulfate (Lo)