Transcriptome sequencing identifies tuberous root development related genes in sweet potato

To screen genes related to the development of sweet potato tuberous roots, the high throughput sequencing of different stages of sweet potato tuberous roots was performed. The fibrous roots (FR; roots at 20 dap), developing tuberous roots (DR; roots at 60 dap) and mature tuberous roots (MR; roots at 120 dap) of Ipomoea batatas (L.) Taizhong 6 and MBP3 overexpressed lines were used for transcriptome analysis. Totally, we identified 5488 differentially expressed genes between different stage tuberous roots of Taizhong6 and 14312 differentially expressed genes between the tuberous roots of Taizhong6 and MBP3 overexpressed lines, by calculating the gene FPKM in each sample and conducting differential gene analysis. This study provides a foundation for the mechanism analysis of sweet potato tuberous root development. The Ipomoea batatas (L.) Taizhong 6 and MBP3 overexpressed plants were planted in a greenhouse at Jiangsu Normal University. The fibrous roots (FR; roots at 20 dap), developing tuberous roots (DR; roots at 60 dap) and mature tuberous roots (MR; roots at 120 dap) of Taizhong 6 were collected and used for RNA extraction and transcriptome analysis. To screen tuberous root formation- and development-related genes, transcriptome sequencing of sweet potato roots in different periods (FR, DR and MR) was performed.