A Single-Cell Resolution Atlas of expanding hematopoietic organ in zebrafish

To profile the developmental landscape of fetal HSPCs and their local niche, here, by using single-cell RNA-sequencing, we decoded the expanding hematopoietic organ in zebrafish Overall design: The tails of 3.5 dpf and 4.5 dpf Tg (kdrl:mCherry/CD41:GFP) embryos were collected and digested with 2.5% trypsin for dissociation.The 50,000 kdrl:mCherry+ cells plus 50000 CD41:GFP+ cells and double negative cells were isolated by fluorescence activated cell sorting (FACS) for 10× Genomics analysis (CapitalBio Technology Corporation). Please note that the GSE120503 (GSM3402061 55hpf CHT single cell RNA seq data) raw data been re-analyzed in the current study.