A Single-Cell Resolution Atlas of expanding hematopoietic organ in zebrafish
收藏NIAID Data Ecosystem2026-03-12 收录
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To profile the developmental landscape of fetal HSPCs and their local niche, here, by using single-cell RNA-sequencing, we decoded the expanding hematopoietic organ in zebrafish Overall design: The tails of 3.5 dpf and 4.5 dpf Tg (kdrl:mCherry/CD41:GFP) embryos were collected and digested with 2.5% trypsin for dissociation.The 50,000 kdrl:mCherry+ cells plus 50000 CD41:GFP+ cells and double negative cells were isolated by fluorescence activated cell sorting (FACS) for 10Ã Genomics analysis (CapitalBio Technology Corporation). Please note that the GSE120503 (GSM3402061 55hpf CHT single cell RNA seq data) raw data been re-analyzed in the current study.
创建时间：
2021-04-29
