RNA sequencing analysis of human embryonic stem cells and axial progenitors

We analysed the transcriptomes of human embryonic stem cells (hESCs) and in vitro derived axial progenitors. Overall design: RNA sequencing of triplicate samples of undifferentiated hESCs grown in Essential 8 medium and Geltrex and axial progenitors generated after a 3 day culture of hPSCs on fibronectin in the presence of bFGF (20 ng/ml) and the WNT agonist/GSK3 inhibitor CHIR99021