Analysis of essential genomic components through determination of transposon insertion sites by deep sequencing of transposon insertion junctions (Tn-Seq) from hyper-saturated 1,000,000 transposon ins
Additional file 3: Culturability-related ‘essential’ L. crescens genes encoding hypothetical proteins that are either secreted or are membrane localized.
Transposon Sequencing (Tn-Seq) is a high-throughput technique that utilizes transposon mutant libraries to assess gene fitness or essentiality under specific conditions potentially identifying novel t