Analysis of GLUT4 dispersal in cultured adipocytes

Insulin stimulates glucose transport in muscle and adipocytes. This is achieved by regulated delivery of intracellular glucose transporter (GLUT4)-containing vesicles to the plasma membrane where they dock and fuse, resulting in increased cell surface GLUT4 levels. Recent work identified a potential further regulatory step, in which insulin increases the dispersal of GLUT4 in the plasma membrane away from the sites of vesicle fusion. EFR3 is a scaffold protein that facilitates localisation of phosphatidylinositol 4-kinase type IIIalpha to the cell surface. Using direct stochastic reconstruction microscopy, dSTORM, we show that EFR3 knockdown impairs insulin stimulated GLUT4 dispersal in the plasma membrane. Enclosed here are the dSTORM datasets and associated analyses for all experiments, presented as a single folder for each experiment. Please also refer to 'dSTORM imaging files for EFR3.. ' record for additional files and analyses.