Lysyl hydroxylase 1 deficiency exacerbates the alterations of gene transcriptional profile of abdominal aorta in angiotensin II induced abdominal aortic aneurysm

Lysyl hydroxylase 1 (LH1) plays an important role in hydroxylation of lysyl residuel in Xaa-Lys-Gly. The hydroxylysine residues serve as sites of attachment for carbohydrate units which are essential for the formation of intra- and intermolecular collagen crosslinks. To gain mechanistic insights into the effects of LH1 deficiency on abdominal aortic aneurysm (AAA) formation, a whole transcriptomic analysis of abdominal aorta were performed using RNA-seq. The abdominal aorta of mice for RNA-seq were acquired at day 14 after angiotensin II infusion in order to provide the mechanistic or causal evidence of a direct participatory role of LH1 to the effects of AAA. Overall design: 20 samples. There are 4 groups: WT-Saline group, wild-type mice on C57BL/6 background were infused with saline by implanting osmotic pumps subcutaneously for 14 days. WT-AngII group, wild-type mice on C57BL/6 background were infused with angiotensin II (1000 ng/kg per minute) by implanting osmotic pumps subcutaneously for 14 days. LH1 KO-Saline group, LH1 knock-out mice on C57BL/6 background were infused with saline by implanting osmotic pumps subcutaneously for 14 days. LH1 knock-out-AngII group, LH1 knock-out mice on C57BL/6 background were infused with angiotensin II (1000 ng/kg per minute) by implanting osmotic pumps subcutaneously for 14 days.