Table 1. Influences of mutations on secreted PLA2 (PLA2) activity.
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ND means non-detected.aNucleotide numbers are based on the sequence of the J35 isolate (GenBank accession no. AY386330).bEnzyme activity was measured by a colorimetric assay kit (Cayman Chemical). Protein concentrations tested: recombinant proteins VP1u(WT, P133R, D154A, H153A, Y157F, Y168F, D175A, D195A, Y157F/Y168F, Y157F/D175A, K162R, and A207Y), 5 µg; bee venom, 10 ng.
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2015-12-02
