m6A-eCLIP analysis of mouse BMDM. m6A-eCLIP analysis of mouse BMDM

N6-methyladenosine (m6A) is the most abundant mRNA modification in mammalian cells, mediated co-transcriptionally by a methyltransferase ‘writer’ complex containing METTL3 as the S-adenosyl methionine (SAM)-binding subunit as well as adaptors such WTAP and ZC3H13. Overall design: To profile m6A mRNA modification sites transcriptome-wide with single nucleotide resolution, we performed enhanced crosslinking and immunoprecipitation (m6A-eCLIP) in mouse BMDMs. All samples were treated with LPS for 3h. This dataset is part of our larger project which also contains human samples. contributor: Eclipse BioInnovations Inc, San Diego CA