mRNA Transcriptase's Analyses That Cotton fruit branch Development Status after silenced GhBRC1 gene byVIGS

Purpose: found out the regulated genes of nulliplex-branch and its forming molecular mechanism Methods: the GhBRC1 genes of nulliplex branch and short branch cotton are silenced by VIGS, and then the shoot apical mRNA of controls and treated were sequenced, in four repeats, using Illumina HiSeq 2000. Results: we found 3519 and 17 differnent expressed genes in nulliplex-branch and short branch cotton, respectively. Conclusions: Our study represents the genes control development of lateral branch. Overall design: The GhBRC1 genes of nulliplex branch and short branch cotton are silenced by VIGS, and then the shoot apical mRNA of controls and silenced were sequenced, in four repeats, using Illumina HiSeq 2000.