miRNA high-throughput sequencing of human prostate cancer cell lines P69, M12, M2182. Homo sapiens

Purpose: Next-generation sequencing (NGS) of miRNA expression in cancer cell lines provides a path for analysis of dysregulated pathways and potential treatment of dysregulation. The goals of this study are to compare NGS-derived miRNA expression data in a non-tumorigenic prostate cell line (P69) to its metastatic derivative cell lines M12 and M2182. Overall design: small RNA sequencing of duplicate extractions of the benign human prostate cell line P69, its metastatic, tumorigenic derivative cell lines M2182 and M12, and a non-metastatic subline developed from M12 using microcell-mediated chromosomal transfer.