RNAseq of irradiated and non-irradiated hemichordate larvae and juveniles

To further characterize the molecular characteristics of proliferative cells in Schizocardium californicum, we exploited the sensitivity of proliferative cells to irradiation. To do this, we treated larvae and juveniles with irradiation. Three days after treatment animals looked morphologically the same as controls, but EdU incorporation was eliminated in both larvae and juveniles. We extracted total RNA from this same stage of three days post-irradiation from 5 pooled individuals in three independent biological replicates and made RNA sequencing libraries. 5 individuals at each stage, larva or juvenile, were treated with 120gy and 200gy respectively. RNA was extracted for three biological replicates.