Whole-genome re-sequencing of EMS induced mutants and wild-type controls to identify mutant specific variants conferring the pendant awn trait

gDNA from mutant and wild-type plants was quality-controlled, normalized (50–250 ng), and used for library preparation. Three libraries per sample were generated, equimolarly pooled, and sequenced on a NovaSeq6000 S2 flow cell (paired-end, 2 × 151 bp, 10 bp index, ~20× coverage) to identify mutant-specific variants associated with the phenotype.