In situ spatial transcriptomic analysis of human skeletal muscle using the Xenium platform

This dataset comprises high-resolution in situ spatial transcriptomic data of human skeletal muscle obtained using the Xenium platform (10x Genomics). Samples from the vastus lateralis muscle of two individuals—a 19-year-old male (Sample_2) and an 85-year-old female with sarcopenia (Sample_1) —were analyzed to investigate transcriptomic heterogeneity.

The dataset includes:

Spatially resolved transcriptomic profiles targeting 377 genes using the Human Multi-Tissue and Cancer Panel.

High resolution post-Xenium H&E images of analysed muscle sections.

High resolution images of serial muscle sections stained against MyHC type 1 (Bad5 Antibody) and MyHC type 2a and 2x (SC71 Antibody).

This dataset is shared to promote further exploration of skeletal muscle biology and the development of advanced spatial transcriptomic techniques.

For more details on methods and analysis, refer to the associated manuscript.