Supplementary Material for: Body Composition and Circulating Polyunsaturated Fatty Acids at Age 6 Years: A Longitudinal Pilot Study

Maternal polyunsaturated fatty acid (PUFA) status during pregnancy may influence birth outcomes and offspring adiposity during childhood. Cord blood PUFA levels associate positively with maternal PUFA and negatively with the newborn’s abdominal adiposity. However, longitudinal, prospective studies consistently evaluating maternal and cord blood and PUFA levels in childhood and their association with the child’s body composition are so far lacking. In a population of 16 apparently healthy children born appropriate for gestational age and followed longitudinally since birth, we assessed circulating PUFA (by gas chromatography) in maternal, cord, and peripheral blood at age 6 years and studied their correlation with body composition (by absorptiometry) and endocrine-metabolic variables at age 6 years. No associations were found among parameters of body composition and endocrine-metabolic variables at age 6 years and maternal, cord blood or circulating PUFA in peripheral blood. Maternal levels of <i>n</i>-6 linoleic acid, total <i>n</i>-6, and the ratio of <i>n</i>-6:<i>n</i>-3 correlated with their corresponding PUFA levels at age 6 years. In conclusion, in this pilot study, maternal, cord blood, and children’s circulating PUFA do not appear to have an impact on body composition and endocrine-metabolic status at the age of 6 years. The close association between maternal PUFA and offspring PUFA at that age may reflect a similarity in nutritional habits in the mother and child.