Gene expression of mouse brain and mouse microglia in NRROS WT and NRROS KO animals

Mice deficient in NRROS exhibit neurological phenotype similar to ALS. Our previous work showed that in phagocytes, NRROS regulates the degradation of Nox2, the enzymatic component of the NADPH oxidase that generates reactive oxygen species. NRROS is expressed in phagocytes, including macrophages, neutrophils and microglia but not neurons. Keywords: Expression profiling by high throughput sequencing To help understand the mechanism of the neurological phenotype caused by loss of NRROS, we looked at NRROS WT (n=5) vs. NRROS KO (n=5) in brains, and NRROS WT (n=5) vs. NRROS KO (n=5) in microglia tissue. Samples were sequenced by the Illumina HiSeq 2500 Sequencing System.NRROS基因缺陷小鼠表现出与肌萎缩侧索硬化症（ALS）相似的神经表型。我们此前的研究表明，在吞噬细胞中，NRROS可调控Nox2的降解——Nox2是产生活性氧的烟酰胺腺嘌呤二核苷酸磷酸氧化酶（NADPH oxidase）的酶促组分。NRROS仅在吞噬细胞（包括巨噬细胞、中性粒细胞和小胶质细胞）中表达，而不在神经元中表达。 关键词：高通量测序表达谱分析 为阐明NRROS缺失引发神经表型的分子机制，我们分别对脑组织中的NRROS野生型（WT，n=5）与NRROS敲除型（KO，n=5）样本，以及小胶质细胞组织中的NRROS野生型（WT，n=5）与NRROS敲除型（KO，n=5）样本进行了测序分析。所有样本均通过Illumina HiSeq 2500测序系统完成测序。