Figure S1 - PPARγ Negatively Regulates T Cell Activation to Prevent Follicular Helper T Cells and Germinal Center Formation

Characterization of lymphocyte populations in CD4-PPARγKO mice. (A-D) Thymic and (E-H) splenic CD4+, CD8+, and Foxp3+ populations from 6- to 8-week-old female wild type and CD4-PPARγKO mice were analyzed by flow cytometry. Total CD4+ or CD8+ cells were gated from live cells while Foxp3+ cells were gated from CD4+ T cells. (I, J) Splenic B and NK cells, which were gated from live cells, and (K, L) CD62L and CD44 populations gated from CD4+ T cells of 6- to 8- week-old female littermate control (Cre-) and CD4-PPARγKO (Cre+) mice were analyzed by flow cytometry. Values represent the mean ± SEM. *P<0.05. (DOCX)